Isoflavones in the rutaceae family: twenty selected representatives of the genera Citrus, Fortunella, Poncirus, Ruta and Severinia
Identifieur interne : 002135 ( Main/Exploration ); précédent : 002134; suivant : 002136Isoflavones in the rutaceae family: twenty selected representatives of the genera Citrus, Fortunella, Poncirus, Ruta and Severinia
Auteurs : Radka Koblovská [République tchèque] ; Zuzana Macková [République tchèque] ; Michaela Vítková [République tchèque] ; Ladislav Kokoška [République tchèque] ; Bo Ivoj Klejdus [République tchèque] ; Old Ich Lap K [République tchèque]Source :
- Phytochemical Analysis [ 0958-0344 ] ; 2008-01.
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Abstract
Enzyme‐linked immunosorbent assays in combination with semi‐preparative high‐performance liquid chromatography (HPLC) and analytical HPLC with mass spectroscopy in the selective ion monitoring mode were used for the determination of selected isoflavones, daidzein, genistein, biochanin A and their homologues, in 20 representatives of the Rutaceae family. Species belonging to five genera were studied, namely Citrus, Fortunella, Poncirus, Ruta and Severinia. The enzyme immunoassays used were based on polyclonal antibodies raised against isoflavonoid conjugates with bovine serum albumin (BSA), namely biochanin A‐7‐BSA, daidzein‐7‐BSA, daidzein‐4′‐BSA, genistein‐7‐BSA and genistein‐4′‐BSA. Aglycones as well as glycosides were detected, and methoxyisoflavones appeared to be more abundant than hydoxyisoflavones. The content of individual isoflavonoids ranged from 0 to 2.6 mg/kg (dry weight); the sum of all measured substances reached up to 5.9 mg. Copyright © 2007 John Wiley & Sons, Ltd.
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DOI: 10.1002/pca.1016
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Species belonging to five genera were studied, namely Citrus, Fortunella, Poncirus, Ruta and Severinia. The enzyme immunoassays used were based on polyclonal antibodies raised against isoflavonoid conjugates with bovine serum albumin (BSA), namely biochanin A‐7‐BSA, daidzein‐7‐BSA, daidzein‐4′‐BSA, genistein‐7‐BSA and genistein‐4′‐BSA. Aglycones as well as glycosides were detected, and methoxyisoflavones appeared to be more abundant than hydoxyisoflavones. The content of individual isoflavonoids ranged from 0 to 2.6 mg/kg (dry weight); the sum of all measured substances reached up to 5.9 mg. Copyright © 2007 John Wiley & Sons, Ltd.</div></front></TEI><affiliations><list><country><li>République tchèque</li></country><region><li>Bohême centrale</li><li>Moravie</li></region><settlement><li>Brno</li><li>Prague</li></settlement></list><tree><country name="République tchèque"><region name="Bohême centrale"><name sortKey="Koblovska, Radka" sort="Koblovska, Radka" uniqKey="Koblovska R" first="Radka" last="Koblovská">Radka Koblovská</name></region><name sortKey="Klejdus, Bo Ivoj" sort="Klejdus, Bo Ivoj" uniqKey="Klejdus B" first="Bo Ivoj" last="Klejdus">Bo Ivoj Klejdus</name><name sortKey="Kokoska, Ladislav" sort="Kokoska, Ladislav" uniqKey="Kokoska L" first="Ladislav" last="Kokoška">Ladislav Kokoška</name><name sortKey="Lap K, Old Ich" sort="Lap K, Old Ich" uniqKey="Lap K O" first="Old Ich" last="Lap K">Old Ich Lap K</name><name sortKey="Mackova, Zuzana" sort="Mackova, Zuzana" uniqKey="Mackova Z" first="Zuzana" last="Macková">Zuzana Macková</name><name sortKey="Vitkova, Michaela" sort="Vitkova, Michaela" uniqKey="Vitkova M" first="Michaela" last="Vítková">Michaela Vítková</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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